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Whoever tells you that the first kill is the hardest is right yet sadly mistaken.
The first thing you kill is not the unfortunate target or the guards around him. The first kill is that part of your conscious that whispers in your mind.
Whispers of how the guard you killed not an hour ago probably has a family out there, probably has someone who cared about him.
It seems that Altair wasn’t able to kill that part after all.