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GLaDOS' Human Experiment Zero by padded_bandit
Fandoms: Portal (Valve Video Games), Half-Life (Video Games), Original Work
24 Feb 2026
Tags
Summary
!! Gender-Neutral Reader-Insert !!
Y/N is a scientist who works at Aperture Science, and is testing the newest prototype of the ASHPD. But due to their dismissal of protocol during previous tests, Aperture higher-ups assigned GLaDOS to supervise Y/N, so she will enforce their compliance. Obsessed with human testing, she spares Y/N during her Neurotoxin killing spree of the corrupt human workers, GLaDOS kept Y/N as her research subject, seeing if with her supervision and parenting, she could raise a human to be what she considers morally correct.
GLaDOS treats Y/N as an infant, slowly removing all of their agency and autonomy, to create the perfect test subject for operant conditioning."Accidents happen," GLaDOS crooned, as a metallic arm delicately pinched the onesie’s sodden crotch and gave it a little shake. Y/N’s thighs pressed together instinctively, the movement making the mess shift in a way that made their cheeks burn hotter than any paddle strike. "But big accidents?" The arm retracted, returning with a fresh diaper—this one easily tripled the thickness of the last, its outer plastic printed with tiny pink crowns. "Those require special containment."